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(A) Transmission electron microscopy (TEM) image of PEI-LSP-RA-PLGA, (B) Scanning electron microscopy (SEM) image of freeze-dried PEI-LSP-RA-PLGA, (C) Stability photographs, (D) Particle size change graph, (E) Zeta potential and PDI variation graph, (F) Loading efficiency of LSP, RA, and <t>H9N2</t> in PEI-LSP-RA-PLGA, (G) In vitro release profile of H9N2.
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(A) Transmission electron microscopy (TEM) image of PEI-LSP-RA-PLGA, (B) Scanning electron microscopy (SEM) image of freeze-dried PEI-LSP-RA-PLGA, (C) Stability photographs, (D) Particle size change graph, (E) Zeta potential and PDI variation graph, (F) Loading efficiency of LSP, RA, and <t>H9N2</t> in PEI-LSP-RA-PLGA, (G) In vitro release profile of H9N2.
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(A) Transmission electron microscopy (TEM) image of PEI-LSP-RA-PLGA, (B) Scanning electron microscopy (SEM) image of freeze-dried PEI-LSP-RA-PLGA, (C) Stability photographs, (D) Particle size change graph, (E) Zeta potential and PDI variation graph, (F) Loading efficiency of LSP, RA, and H9N2 in PEI-LSP-RA-PLGA, (G) In vitro release profile of H9N2.

Journal: Poultry Science

Article Title: A Novel PLGA-based nano-adjuvant augments mucosal and systemic immunity in chicks through intestinal targeting and IgA production

doi: 10.1016/j.psj.2026.106641

Figure Lengend Snippet: (A) Transmission electron microscopy (TEM) image of PEI-LSP-RA-PLGA, (B) Scanning electron microscopy (SEM) image of freeze-dried PEI-LSP-RA-PLGA, (C) Stability photographs, (D) Particle size change graph, (E) Zeta potential and PDI variation graph, (F) Loading efficiency of LSP, RA, and H9N2 in PEI-LSP-RA-PLGA, (G) In vitro release profile of H9N2.

Article Snippet: The H9N2 antigen (Luoyang Pulike, China) was mixed with the adjuvants PEI-LSP-RA-PLGA, PEI-RA-PLGA, LSP, and Alum (Thermo, USA) at a volume ratio of 1:3 and shaken on a shaker for 30 minutes to prepare PEI-LSP-RA-PLGA/H9N2, PEI-LSP-PLGA/H9N2, LSP/H9N2, and Alum/H9N2.

Techniques: Transmission Assay, Electron Microscopy, Zeta Potential Analyzer, In Vitro

(A) Effects of different experimental groups on H9N2-IgG and H9N2-IgA content on chickens, (B) Effects of different experimental groups of IL-4, IL-5, IL-10, IL-12, and IFN-γ secretion on chickens. a-e Superscript different significant difference ( P < 0.05), n = 5.

Journal: Poultry Science

Article Title: A Novel PLGA-based nano-adjuvant augments mucosal and systemic immunity in chicks through intestinal targeting and IgA production

doi: 10.1016/j.psj.2026.106641

Figure Lengend Snippet: (A) Effects of different experimental groups on H9N2-IgG and H9N2-IgA content on chickens, (B) Effects of different experimental groups of IL-4, IL-5, IL-10, IL-12, and IFN-γ secretion on chickens. a-e Superscript different significant difference ( P < 0.05), n = 5.

Article Snippet: The H9N2 antigen (Luoyang Pulike, China) was mixed with the adjuvants PEI-LSP-RA-PLGA, PEI-RA-PLGA, LSP, and Alum (Thermo, USA) at a volume ratio of 1:3 and shaken on a shaker for 30 minutes to prepare PEI-LSP-RA-PLGA/H9N2, PEI-LSP-PLGA/H9N2, LSP/H9N2, and Alum/H9N2.

Techniques: